Steven Chu (2014) - You can see a lot by observing: Optical Microscopy 2.0

Steven Chu (2014)

You can see a lot by observing: Optical Microscopy 2.0

Steven Chu (2014)

You can see a lot by observing: Optical Microscopy 2.0

Abstract

Biological research and medicine were transformed by the invention and improvement of the optical microscope. Since the early 1990s, there has been another revolution in optical imaging, and manipulation of individual biological molecules and bio-molecular systems have been demonstrated and applied to a wide variety of systems. Most recently, innovations in “super-resolution” optical imaging, such as STORM and PALM have been used to construct biological images with ~ 10 nm resolution. With bright optical probes and corrections to the slight differences in sensitivity to the CCD or CMOS camera, < 1 nm resolution is possible in biological samples in water.[1] Recent applications of super-resolution imaging to cancer signaling[2], and bio-films[3] will be discussed.

Finally, the development of sub-wavelength micro-spectroscopy in the fingerprint region of the infrared spectra (wavelength = 4 – 12 µm) to observe changes in biological states with 20 nm spatial resolution will be outlined.

[1] Subnanometre single-molecule localization, registration and distance measurements, Alexandros Pertsinidis, Yunxiang Zhang, Steven Chu, Nature 466 , 647–651 (2010).
[2] Ras-GTP Dimers Activate the Mitogen-Activated Protein Kinase (MAPK) Pathway, Xiaolin Nan, Tanja Meyer-Tamgüney, Eric A. Collisson, Li-Jung Lin, Cameron Pitt, Jacqueline Galeas, Sophia Lewis, Joe W. Gray, Frank McCormick, Steven Chu, submitted (2014)
[3] Molecular Architecture and Assembly Principles of Vibrio cholerae Biofilms, Veysel Berk, Jiunn C. N. Fong, Graham T. Dempsey, Omer N. Develioglu, Xiaowei Zhuang, Jan Liphardt, Fitnat H. Yildiz, Steven Chu, Science 337, pp. 236-239 (2012)

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